A novel approach for preparing disulfide-rich peptide-KLH conjugate applicable to the antibody production.
Katayama H, Mizuno R, Mita M.
Biosci Biotechnol Biochem. 2019 Oct; 83(10):1791-1799. doi: 10.1080/09168451.2019.1618696. Epub 2019 May 21.

 

Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer
Juan José Mora Román 1, Miguel Del Campo 1, Javiera Villar 1, Francesca Paolini 2, Gianfranca Curzio 2, Aldo Venuti 2, Lilian Jara 3, Jorge Ferreira 4, Paola Murgas 5, Alvaro Lladser 5, Augusto Manubens 6, María Inés Becker 1 6

J Immunol Res. 2019 Jan 20;2019:7076942. doi: 10.1155/2019/7076942. eCollection 2019.

 

TLR4, but Neither Dectin-1 nor Dectin-2, Participates in the Mollusk Hemocyanin-Induced Proinflammatory Effects in Antigen-Presenting Cells From Mammals
José M Jiménez 1, Michelle L Salazar 1, Sergio Arancibia 1, Javiera Villar 1, Fabián Salazar 1 2, Gordon D Brown 2, Ed C Lavelle 3, Luisa Martínez-Pomares 4, Jafet Ortiz-Quintero 5, Sergio Lavandero 5, Augusto Manubens 6, María Inés Becker 1 6

Front Immunol. 2019 May 31;10:1136. doi: 10.3389/fimmu.2019.01136. eCollection 2019.

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Anti-cancer properties of gastropodan hemocyanins in murine model of colon carcinoma
Vera Gesheva, Stela Chausheva, Nikolina Mihaylova, Iliyan Manoylov, Lyuba Doumanova, Krassimira Idakieva, Andrey Tchorbanov 1

Front Immunol. 2019 May 31;10:1136. doi: 10.3389/fimmu.2019.01136. eCollection 2019.

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Monitoring of dynamic changes in Keyhole Limpet Hemocyanin (KLH)-specific B cells in KLH-vaccinated cancer patients
Florian Wimmers 1, Nienke de Haas 1, Anja Scholzen 2, Gerty Schreibelt 1, Elles Simonetti 3, Marc J Eleveld 3, Huberdina M L M Brouwers 4, Marjo Beldhuis-Valkis 4, Irma Joosten 4, Marien I de Jonge 3, Winald R Gerritsen 5, I Jolanda M de Vries 1 5, Dimitri A Diavatopoulos 3, Joannes F M Jacobs 4

Clinical Trial Sci Rep. 2017 Mar 7;7:43486. doi: 10.1038/srep43486.

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Marine-Derived Pharmaceuticals - Challenges and Opportunities
Ulrike Lindequis

Biomol Ther (Seoul). 2016 Nov 1;24(6):561-571. doi: 10.4062/biomolther.2016.181.

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Keyhole limpet haemocyanin - a model antigen for human immunotoxicological studies
Ashwin Swaminathan 1, Robyn M Lucas, Keith Dear, Anthony J McMichael

Published online 2014 Oct 20. doi: 10.1111/bcp.12422

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Mollusk Hemocyanins as Natural Immunostimulants in Biomedical Applications

María Inés Becker, Sergio Arancibia, Fabián Salazar, Miguel Del Campo and Alfredo De Ioannes

Published: May 29th 2014

DOI: 10.5772/57552

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A novel immunomodulatory hemocyanin from the limpet Fissurella latimarginata promotes potent anti-tumor activity in melanoma
Sergio Arancibia 1, Cecilia Espinoza 1, Fabián Salazar 1, Miguel Del Campo 1, Ricardo Tampe 1, Ta-Ying Zhong 1, Pablo De Ioannes 1, Bruno Moltedo 1, Jorge Ferreira 2, Ed C Lavelle 3, Augusto Manubens 4, Alfredo E De Ioannes 4, María Inés Becker 5

PLoS One. 2014 Jan 23;9(1):e87240. doi: 10.1371/journal.pone.0087240. eCollection 2014.

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Intracutaneous and intravesical immunotherapy with keyhole limpet hemocyanin compared with intravesical mitomycin in patients with non-muscle-invasive bladder cancer: results from a prospective randomized phase III trial

Rianne J M Lammers 1, Wim P J Witjes, Maria H D Janzing-Pastors, Christien T M Caris, J Alfred Witjes

Clinical Trial: J Clin Oncol. 2012 Jun 20;30(18): 2273-9. doi: 10.1200/JCO.2011.39.2936. Epub 2012 May 14.

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The refined structure of functional unit h of keyhole limpet hemocyanin (KLH1-h) reveals disulfide bridges
Elmar Jaenicke 1, Kay Büchler, Heinz Decker, Jürgen Markl, Gunnar F Schröder

IUBMB Life. 2011 Mar;63(3):183-7. doi: 10.1002/iub.435.

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Concholepas hemocyanin biosynthesis takes place in the hepatopancreas, with hemocytes being involved in its metabolism
Augusto Manubens 1, Fabián Salazar, Denise Haussmann, Jaime Figueroa, Miguel Del Campo, Jonathan Martínez Pinto, Laura Huaquín, Alejandro Venegas, María Inés Becker

Cell Tissue Res. 2010 Dec; 342(3):423-35. doi: 10.1007/s00441-010-1057-6. Epub 2010 Nov 3.

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Keyhole limpet hemocyanin induces the activation and maturation of human dendritic cells through the involvement of mannose receptor
Pietro Presicce 1, Adriano Taddeo, Antonietta Conti, Maria Luisa Villa, Silvia Della Bella ol Immunol.

2008 Feb;45(4): 1136-45. doi: 10.1016/j.molimm.2007.07.020. Epub 2007 Sep 4.

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Structure and function of haemocytes in two marine gastropods, Megathura crenulata and Aplysia californica 
Gary G. Martin, Christopher T. Oakes, Heather R. Tousignant, Heather Crabtree, Rachel Yamakawa
Journal of Molluscan Studies, Volume 73, Issue 4, November 2007, Pages 355–365,     

Published: 15 October 2007  https://doi.org/10.1093/mollus/eym032

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The effect of captivity and diet on KLH isoform ratios in Megathura crenulata
Frank R Oakes 1, Sarah McTee, John McMullen, Carolynn S Culver, Daniel E Morse

Comparative Study Comp Biochem Physiol A Mol Integr Physiol. 2004 Jun;138(2):169-73. doi: 10.1016/j.cbpb.2004.03.009.

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Localization and identification of Schistosoma mansoni /KLH-crossreactive components in infected mice
Cecilia Thors 1, Ewert Linder

J Histochem Cytochem. 2003 Oct; 51(10): 1367-73. doi: 10.1177/002215540305101013.

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Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal (beta1-6)Man-motifs
Tomofumi Kurokawa 1, Manfred Wuhrer, Günter Lochnit, Hildegard Geyer, Jürgen Markl, Rudolf Geyerur

J Biochem. 2002 Nov; 269(22):5459-73. doi: 10.1046/j.1432-1033.2002.03244.x.

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Keyhole limpet hemocyanin (KLH): a biomedical review

J R Harris 1, J Markl

Micron. 1999 Dec;30(6):597-623. doi: 10.1016/s0968-4328(99)00036-0.

DOI: 10.1016/s0968-4328(99)00036-0

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Mass determination, subunit organization and control of oligomerization states of keyhole limpet hemocyanin (KLH)
S M Söhngen 1, A Stahlmann, J R Harris, S A Müller, A Engel, J Markl

Eur J Biochem. 1997 Sep 1; 248(2):602-14. doi: 10.1111/j.1432-1033.1997.00602.x.